Application of soft tissue augmentation in labial contour aesthetics of anterior dental implants / 口腔疾病防治

@#After tooth extraction, labial contour collapses due to inevitable physiologic bone remodeling. To achieve optimal outcomes for pink esthetic treatment at anterior implant sites, bone or soft tissue augmentation has been advocated to maintain or reconstruct the labial tissue contour. When choosing soft tissue augmentation for esthetic restoration, it is necessary to strictly grasp the indications for surgery. Soft tissue augmentation to maintain or reconstruct the labial tissue contour could be considered in patients with healthy soft tissue and no bone defects or only mild horizontal bone defects. In immediate, early and late implant placement, the timing of soft tissue augmentation may vary. In immediate implantation, the labial bone plate is intact, so it is highly recommended to simultaneously manage soft tissue during implant placement. However, patients may have large bone defects with early or late implant placement. The risk of augmenting bone and soft tissue simultaneously is likely too high, and bone augmentation surgery is often performed at the first stage while soft tissue augmentation surgery is performed at the second stage. Therefore, soft tissue surgery is often carried out simultaneously with abutment connection. Currently, soft tissue augmentation is achieved mostly with adjacent autologous soft tissue grafts, such as free gingival grafts, subepithelial connective tissue grafts or pedicle palatal flaps, which are often accompanied by a second surgical area. The replacement of autogenous soft tissue grafting with new biological materials will become an inevitable trend. In this article, we analyze and summarize the indications, timing and different methods of soft tissue augmentation to maintain and reconstruct the labial contour.

Coverage Of Multiple Maxillary Gingival Recessions Using Coronally Advanced Flap With Subepithelial Connective Tissue Graft: A Case Report

Background:This case report describes treatment of multiple gingival recession with subgingival connective tissue graft and coronally advanced flap technique in treatment of maxillary teeth. Material And Methods:Connective tissue grafting wasdone in relation to upper right second premolar, first premolar, canine, right central incisor, left central incisor and left lateral incisor (#15,#14,#13,#11,#21,#22). A split thickness flap was elevated without disturbing periosteum in this region. The area between canine and second premolar was selected to harvest the graft. The graft was placed on the recipient bed and suturing was done. Result:Predictable root surface coverage could be obtained with use of coronally advanced flap and subepithelial connective tissue graft. Conclusion:Subepithelial connective tissue graft along with coronally advanced flap still stand as a gold standard treatment for gingival recession coverage.

Construction of an experimental millerⅢ gingival retraction animal model in beagle dogs / 口腔疾病防治

Objective @#To construct a Miller class Ⅲ gingival recession animal model and to lay the foundation for exploring the treatment of Miller class Ⅲ gingival recession. @*Methods@#Two adult male beagle dogs were selected, and four teeth from each beagle dog were selected to establish an experimental Miller class Ⅲ gingival recession model. The root surface was revealed by removing the soft and hard tissues of the buccal side. The success of the model was determined by measuring the vertical gingival retraction (VGR), horizontal retraction (HGR), keratosis tissue width (KTW), gingival tissue thickness (GTT), and probing depth (PD) at 1, 2, 4, 6, and 8 weeks after modeling. @*Results@#After observing the clinical indexes, the PDs before and after the modeling were all smaller than 3 mm and no deep-period pockets were formed. The VGR before modeling was 0 mm, and the VGR range after modeling was 5-6.38 mm. A comparison of the before and after modeling results showed that this difference was statistically significant (P < 0.05). The postoperative VGR results were grouped according to timepoint. A comparison between the two groups showed that the differences at 2, 4, 6 and 8 weeks postoperatively were not statistically significant (P > 0.05). The HGR before the modeling was 0 mm, and the HGR fluctuated around 10.5 mm after the modeling, and this difference was statistically significant (P < 0.05). The HGR results were grouped by timepoint after surgery, and a one-way analysis of showed that the differences between the two groups were not statistically significant (P > 0.05). The KTW range before modeling was 6~9 mm, and it fluctuated around 2 mm after modeling, and this difference was statistically significant (P < 0.05). The KTW results were grouped by timepoint after surgery, and they indicated that significant differences did not occur between the groups postoperatively (P > 0.05). The pre-modeling GTT was 1.5 mm, and the GTT range after modeling was 1.5-2 mm. The preoperative and postoperative GTT results were grouped by timepoint, and the results showed that significant differences did not occur between 1 week and 2 weeks after surgery (P = 0.123), although a statistically significant difference was observed at 1 week postoperatively between this group and the other groups (P < 0.05).@*Conclusion@#The method used in this experiment can successfully build a Miller class III gingival recession animal model, and the model remains stable after wound healing.

Enxerto de tecido conjuntivo subepitelial e retalho coronal em recessões gengivais múltiplas / Subepithelial connective tissue graft and coronal fl ap in multiple gingival recessions

Algumas das principais indicações para o recobrimento de superfícies radiculares expostas por recessão gengival compreendem a hipersensibilidade dentinária cervical e o tratamento de defeitos estéticos. Este trabalho descreveu o tratamento de recessão gengival múltipla classe III de Miller em um periodonto delgado, após 36 meses de uma intervenção cirúrgica com enxerto gengival livre. O tratamento cirúrgico periodontal proposto foi o recobrimento radicular por meio de enxerto de tecido conjuntivo subepitelial associado a retalho reposicionado coronalmente na região vestibular dos elementos 31, 41 e 42. Após 90 dias, foi possível observar o recobrimento parcial da recessão, aumento da espessura tecidual e o controle do biofi lme pelo paciente, promovendo maior proteção da região e redução do risco de recidiva das recessões. Diante da resolução do caso clínico apresentado, pôde-se concluir que a técnica do enxerto de tecido conjuntivo subepitelial associado ao retalho reposicionado coronalmente, quando bem indicada e realizada, pode ser empregada com sucesso em áreas de recessões múltiplas e periodonto com espessura reduzida.

Some of the main indications for the root coverage of gingival recessions include cervical dentin hypersensitivity and treatment of aesthetic defects. This manuscript describes the treatment of a Miller's Class III multiple gingival recession 36 months after a free gingival graft surgical procedure. The periodontal surgical treatment proposed was the subepithelial connective tissue graft associated with a coronally repositioned fl ap at vestibular region of elements 31, 41 and 42. After 90 postoperative days, it was possible to observe an increase on tissue thickness and biofi lm control by the patient, promoting a greater protection of the region and reducing the risk of recession recurrence. It can be concluded that the subepithelial connective tissue graft technique associated with the coronal repositioned fl ap, when well indicated and performed, can be successfully used in multiple recessions and periodontal regions with reduced thickness.

Optimización del injerto conectivo subepitelial en la cobertura de recesiones gingivales múltiples contiguas: presentación de un caso clínico / Optimization of the subephitelial connectivetissue graft to treat adjacent multiple gingivalrecessions. Clinical case presentation

Objetivo: describir, mediante un caso clínico, una técnica quirúrgica para la optimización del injerto conectivo libre subepitelial en la cobertura de recesiones gingivales contiguas múltiples. Caso clínico: Una paciente de sexo femenino, de 57 años de edad, concurrió a la consulta con recesiones clase III de Miller, abfracciones, erosiones y malposición. El procedimiento quirúrgico elegido fue la técnica de desplazado coronal con incisiones oblicuas. Se realizó la toma de un injerto conectivo subepitelial y se lo optimizó en seis porciones trian-gulares, de manera tal que cada una abarcara una recesión desde el 13 hasta el 23. Se efectuó el seguimiento de la cicatrización y de la estabilidad de la cobertura radicular a los 90 días.Conclusión: Este nuevo abordaje quirúrgico es una alternativa válida para cubrir recesiones gingivales múltiples en un solo procedimiento, al poder aprovechar al máximo la utilización de un injerto conectivo libre subepitelial.

Aim: to present the surgical resolution of a clinical caseof multiple gingival recessions treated with a novel approach.Case report: A 57 year-old non-smoking female patientwith Miller Class III gingival recessions abfractions, erosionsand tooth malposition came to the consultation. The treatmentthat was chosen consisted in covering the gingival recessionswith a coronal advanced flap with oblique incisions. A subephitelialgingival graft was harvested from the palate andan optimization in triangular portions was made, in order touse one portion for each recession. Healing and stability wererecorded for a 90 days follow-up period.Conclusion: This new surgical approach could be analternative for the treatment of multiple gingival recessionsin a single procedure, as the use of the ILS could be maximized.

Subepithelial connective tissue graft: a case report

Introduction and objective: Marginal tissue recession represents a common condition in Periodontology. Miller's Classes I and II recessions, in which the etiological factors are well diagnosed and eliminated, show great predictability of total coverage when the technique of subepithelial connective tissue graft is used. This technique success has been mainly attributed to the double blood supply for graft's nutrition, originating from the connective tissue of both the periosteum and flap. Case report and conclusion: The authors reported a clinical case in which a Miller's Class I recession was treated by the surgical technique of subepithelial connective tissue graft, obtaining total coverage, eliminating the aesthetic deficiency and the dentin hypersensitivity complained by patient.

Two-step procedure for root coverage using a free gingival graft and a subepthelial connective tissue graft.

Gingival recession is a multifaceted problem, for which several treatment options are available. Both epithelized and subepithelial connective tissue grafts offer predictable solutions for the treatment of gingival recession.The case report involved a 30-year-old man with gingival recession of 8 mm on 41 (Miller's class II recession). Before surgery, full mouth scaling and polishing were performed. Recession height, width, probing depth, clinical attachment level (CAL), and width of the attached gingiva (WAG) were measured at the baseline, two months postoperatively, and six months postoperatively. Four weeks after scaling, the first step of increasing the width of the attached gingiva was carried out, using a free gingival graft. Two months after this step, the subepithelial connective tissue was harvested from the palate and placed in relation to 41, to cover the residual defect.Two months postoperatively, there was a 3 mm gain in WAG and 35% root coverage. Six months postoperatively there was a significant increase in WAG, CAL; and reduction in height and width of recession (root coverage achieved was 75%). These results suggested that this two-stage surgical procedure could be successful for root coverage in case of deep recession and lack of attached gingiva in the mandibular anterior region.

Recobrimento radicular com enxerto de tecido conjuntivo subepitelial em recessões classe III de Miller / Root coverage with subepithelial connective tissue graft in Miller class III recessions

Atualmente, a demanda por cirurgia plástica periodontal para tratamentos de recessões gengivais se tornou rotina, principalmente quando o paciente relata desconfortos como hipersensibilidade ou prejuízo de estética pela exposição radicular. A etiologia é considerada multifatorial, podendo estar presentes fatores predisponentes e/ou desencadeantes. Várias são as técnicas utilizadas para o recobrimento radicular, dentre elas o retalho posicionado coronalmente associado ao enxerto de tecido conjuntivo subepitelial. No caso clínico apresentado, as recessões gengivais foram classificadas como classe III de Miller e o tratamento clínico promoveu ganho de tecido ceratinizado, recobrimento radicular e a redução significativa da hipersensibilidade, que era a queixa principal da paciente, representando assim uma alternativa viável para o tratamento dessas recessões. Considerações sobre fatores biológicos e etiológicos podem influenciar na previsibilidade do recobrimento, sendo fundamental a escolha da melhor alternativa terapêutica, visando minimizar o trauma cirúrgico e favorecer melhores resultados, solucionando problemas estéticos e alterações funcionais.

Currently, the demand for periodontal plastic surgery for treatment of gingival recessions has become routine, especially when the patient reports discomforts as hypersensitivity or prejudice of aesthetics by root exposure. The etiology is considered multifactorial and may be present predisposing factors and trigger. There are several techniques used for root coverage, and among them, the coronally positioned flap associated with subepithelial connective tissue graft. In the clinical case presented, the gingival recessions were classified as class III of Miller and clinical treatment promoted gain of keratinized tissue, root coverage and the significant reduction of hypersensitivity, which was the main complaint of the patient, thus representing a feasible alternative for the treatment of gingival recessions. Considerations about biological and etiological factors may influence the predictability of the coverage, and is important to choose the best therapeutic option, seeking to minimize the surgical trauma and to promote better results, troubleshooting aesthetic and functional changes.

Subepithelial Connective Tissue Graft For Root Coverage - Report Of Two Cases.

Esthetic concerns of the patient have become an essential part of dentistry, especially periodontics. Periodontal plastic surgery is a rapidly emerging field which helps us to meet this criterion. Root coverage can be achieved by various techniques namely pedicle grafts and free soft tissue grafts. This article highlights on case report in which subepithelial connective tissue has been used for root coverage.

Periodontal plastic surgery for cosmetic root coverage: A case report.

Based on clinical observation that gingival recession may occur during orthodontic therapy involving sites that have an “insufficient” zone of gingiva, it is suggested that grafting procedure to be adapted to increase the gingival dimensions preceding the initiation of the orthodontic therapy. But once it is created, should be solved in appropriate way. We report a case treated for post-orthodontic gingival recession. The procedure involved the utilization of subepithelial connective tissue graft (SCTG) combined with coronally advanced flap procedure. At the post-operative follow-up visits the success of the surgical approach was confirmed by the absence of bleeding to probing and periodontal pockets as well as presence of gingival tissue with normal color, texture and contouring. After 12 months of follow-up, the clinical conditions were better than immediate post-operative result with satisfactory root coverage and periodontal health by the process known as creeping attachment. An excellent esthetical outcome was achieved and the patient was satisfied with case resolution.

Evaluation of alteration in mucogingival line location following use of subepithelial connective tissue graft.

Aim and Objective : The aim of this study is to evaluate the positional changes that occur in mucogingival line following the use of subepithelial connective tissue graft (SCTG). Materials and Methods : In 19 Miller class I or II gingival recession defects, distance between mucogingival line (MGL) and cemento-enamel junction, also width of keratinized and attached gingiva, and clinical attachment level were measured. SCTG were used for covering the exposed roots. A fore mentioned parameters were repeated at 3, 6 and 12 months after surgery and alterations were measured. Paired t test was used to analyze the results. Results : MGL had been moved in coronal direction (4.39 ± 0.77 mm on average) during surgical approach. After 1 year, MGL shifted 2.11 ± 0.7 mm apically. In accordance with this apical shift, a significant increase in the width of keratinized and attached gingival width (2.89 ± 0.63 mm and 2.82 ± 0.5 mm, respectively) was seen (P < 0.05). Conclusion : MGL tended to revert back to its original position following the use of SCTG, and this reversion is accompanied with an increase in the keratinized and attached gingival width.

Root coverage with subeptithelial connective tissue grafts / 대한치주과학회지

Marginal tissue recession makes problems like esthetics, root caries, hypersensitivity and plaque accumulation. Request for root coverage is higer than ever, especially esthetic problems involved. So techniques for root coverage hav been developed. There are some kinds of surgical techniques using soft tissue for root coverage. For example, free gingival graft, kinds of pedicle flap, subepithelial connective tissue graft(SCTG), and so on. Subepithelial connective tissue graft has many advantage for root coverage, that is less pain on donor site, good blood supply for graft, and more esthetic result. For this reaseon, this case report was performed to evaluate the effect of root coverage using subepithelial connective tissue graft. Three patients has Miller's class I marginal tissue recession and one patients has Miller's class III marginal tissue recession. The following period is 36.5 month on average. The results are as follows: 1. Root coverage of 100% was obtained in 5 of 6 defects, and 80% was obtained in 1 of 6 defects. The mean root coverage was 96.6% in six cases on 4 patients. 2. The mean root coverage was 3.83mm and mean recession depth decreased from 4mm to 0.16mm. 3. The mean width of clinical attached gingiva increased from 1.5mm to 4mm. The mean width of gained attached gingiva after surgery was 2.5mm. 4. The mean follow up period was 36.5 months. The longest follow up period was 50 months and the shortest follow up period was 22 months. 5. The result that obtained by surgery was stable during follow up period. Within the above results, root coverage with SCTG is an effective procedure to cover marginal tissue recession defect with long term stability.

Avaliação clínica do uso do Alloderm®, enxerto conjuntivo e retalho reposicionado coronariamente no tratamento da recessão gengival / Clinical evaluation of the use of Alloderm®, connective tissue graft and coronally advanced flap in treatment of gengival recession

O objetivo do presente trabalho foi avaliar clinicamente, o enxerto de tecido conjuntivo subepitelial (CO), o Alloderm® (ALL) e o retalho reposicionado coronariamente (RPC) quando utilizados no tratamento da recessão gengival tipo classe I de Miller. Trinta pacientes participaram do estudo. Os pacientes foram distribuídos em três grupos de 10 pacientes. Dez pacientes com idade média de 38.7(±10.3) anos foram tratados pela técnica do RPC, incluindo 18 recessões. Dez pacientes com idade média de 40.2(+/-9.9) anos foram tratados pela técnica do CO, totalizando 23 recessões. Dez pacientes com idade média de 37.7(±11.2) anos foram tratados através da utilização do ALL, totalizando 18 recessões. Os pacientes não poderiam apresentar cárie sobre a superfície radicular exposta, a junção amelocementária deveria ser visível e identificável, não poderia ter sido submetido a nenhum tipo de tratamento cirúrgico nos elementos envolvidos no experimento nos últimos 24 meses e não apresentar bolsa periodontal ou tratamento endodôntico nos elementos dentários candidatos à cobertura das superfícies radiculares expostas. As seguintes mensurações foram realizadas: índice de placa (IP), índice gengival (IG), profundidade de bolsa à sondagem (PBS), nível de inserção à sondagem (NIS), largura de gengiva ceratinizada (GI), profundidade da recessão gengival (PRG), posição da linha muco-gengival (PLMG). As medidas de IP, IG, PBS, NIS, GI, PRG e PLMG, foram executadas com sonda periodontal calibrada nos dias 0, 30, 60, 90, 120 e 180, exceto PBS e NIS que não foram mensuradas no dia 30. Avaliações subjetivas relativas ao resultado estético alcançado foram realizadas pelo operador e pelo paciente no sexto mês pós-operatório. O teste T de Student para amostras pareadas foi utilizado nas análises intragrupos e o ANOVA foi utilizado nas análises entre os grupos (p<0.05). Os resultados mostraram uma cobertura radicular média de 66.8% para o grupo RPC, enquanto a cobertura radicular média dos grupos ALL e CO foi de 75.9% e de 87.9%, respectivamente. Não houve diferença estatisticamente significante entre os valores de cobertura radicular média entre os grupos ALL e CO. Os valores para o grupo CO eram significantemente maiores que o do grupo RPC. Os valores médios de ganho de inserção clínica de 4.2(±0.9) mm e de 3.5(±1.0) mm dos grupos CO e ALL, respectivamente, foram significantemente maiores que os valores de 2.1(±0.7) mm do grupo RPC. O aumento médio de largura de gengiva ceratinizada foi de 1.41(±0.3) mm no grupo CO e de 1.3(±0.2) mm no grupo ALL, não havendo diferença significativa entre os dois grupos. Não houve alteração na largura de gengiva ceratinizada no grupo RPC. Concluindo, as técnicas de CO e ALL apresentaram resultados clínicos semelhantes e superiores ao RPC em relação ao ganho de inserção clínica, aumento na largura de gengiva ceratinizada e cobertura radicular

The aim of the present study was to clinically evaluate the subepithelial connective tissue graft (CTG), Alloderm (ALL) and coronally advanced flap (CAF) in the treatment of gingival recessions Class I of Miller. 30 patients were included in the study, and were divided in 3 groups of 10 patients. 10 patients with mean age of 38.7 (± 10,3) years were treated with CAF, including 18 defects. 10 patients with mean age of 40.2(± 9.9) years were treated with CTG, including 23 defects, while 10 patients with mean age of 37.7(±11.2) were treated with ALL, including 18 gingival recessions. The patients should not have caries over the denuded root surface, the cementoename junction should visible and easily identified, should not have received any surgical treatment during the last 24 months, and not have periodontal pockets or endodontic treatment at the tested teeth. The following measurements were performed: Plaque index (PI), gingival index (GI), probing pocket depth (PPD), probing attachment level (PAL), width of keratinized gingiva (KG), depth of gingival recession (GR) and position of the mucogingival junction (MGJ). PI, GI, PPD, PAL, KG, GR, MGJ were made with a calibrated periodontal probe on days 0, 30, 60, 90, 120 and 180. PPD and PAL were not measured on day 30. Subjective evaluations were performed by the patient and the dentist on day 180. Paired T Test was used to observe differences within the groups in the different time periods, and ANOVA was performed to analyze differences between the 3 surgical procedures (p<0.05). The results showed that the mean root coverage was 66.8% for the CAF group, while the corresponding values for ALL and CTG were 775.9% and 81.4%, respectively. There was no significant difference between the values of ALL and CO, but the mean values for root coverage of CO group were statistically higher than the CAF group. The mean values of clinical attachment gain of 4.2 (± 0.9) mm and 3.5 (± 1.0) mm in CTG and ALL groups, respectively, were statistically higher than the corresponding values for CAF group (2.1 ± 0.7 mm). There was no significant difference between the mean increase of KG of 1.4 (± 0.3) mm in CTG group and 1.3(± 0.2) mm in ALL group. KG was not different in the CAF group. In conclusion, CTG and ALL procedures presented similar and better results than CAF procedure in relation to clinical attachment gain, increase in the width of KG and the % of root coverage

A Clinical Results of Subepithelial Connective Tissue Graft for Root Coverage / 대한치주과학회지

Exposed root surfaces can cause esthetic problems, hypersensitivity, and root caries. Numerous efforts have been tried to cover the recessed root surfaces, and various techniques have been developed and introduced. Among these, subepithelial connective tissue graft which shows high coverage rate in various researches, has the advantage of good color match, less discomfort to the donor site, rich vascularity, and high predictability. Following results were obtained after investigating 6 and 18 months post operatively, 98 cases of subepithelial connective tissue graft from 48 patients who underwent subepithelial connective tissue graft procedure in the department of periodontology, college of dentistry, Yonsei university. 1. The total average root coverage of Miller class I, II & III were 76.2?24% at 6 months follow-up and 75?25.2% at 18 months follow-up with no statistically significant difference between the follow-up periods.(p<0.05) 2. The percentage of teeth showing complete coverage were 41.9% at 6 months follow-up and 39.2% at 18 months follow-up. 3. At 6 months follow-up, Miller classification I showed 84.9?20.7%, class II showed 82.5?17.7%, and class III showed 62.3?24.5% of coverage. In class III recession, statistically significantly less root coverage was observed compared to class I & II.(p<0.05) 4. At 18 months follow-up, Miller classification I showed 92.2?13.5%, class II showed 84.3?17.4%, and class III showed 59.5?24.5% of coverage. In class III recession, statistically significantly less root coverage was observed compared to class I & II.(p<0.05) In conclusion, subepithelial connective tissue graft for class I and II recession can be used as a clinically predictable treatment modality for root coverage.

The application of subepithelial connective tissue graft in the treatment of gingival recession / 实用口腔医学杂志

Objective:To study the effect of subepithelial connective tissue grafting in covering an exposed root surface.Methods:Eight cases with gingival recession on upper and lower anterior teeth and premolars receited connective tissue grafting received connective tissue grafting were presented with 12 months of follow-up.Results:Initial gingival recession averaged 4.3 mm,with a mean probing pocket depth of 2.3 mm.At the 12-month follow-up,a mean of 3.0 mm of root coverage was observed(ie,71% root coverage,range 60% to 80%).Probing pocket depth averaged 1.6 mm,indicating a 3.6 mm gain of clinical attachment(range 2 to 5 mm).Conclusion:The application of connective tissue graft results in predictable root coverage and clinical attachment while maintaining shallow pockets.

Periodontal Wound Healing of the Experimental Subepithelial Connective Tissue Graft in Dogs / 대한치주과학회지

Several methods have been used for regeneration of tissue lost by periodontal disease. Subepithelial connective tissue graft technique, one of the technniques of mucogingival surgery, is used for the regeneration in esthetic problems such as recession, and denuded root coverage. This study is performed to evaluate the healing process and the regeneration and reattachment of periodontal tissue, including the reconstruction of junctional epithelium, and connective tissue. Alveolar defects in five adult dogs were treated with periodontal surgery and were attained by removing the marginal alveolar bone by 4x3mm from CEJ in the labial side of incisors, and root surfaces were planed. The experimental sites were divided into two groups as follows. 1. root planing alone(control group) 2. with connective tissue graft(Experimental Group) In the two groups flaps were positioned and sutured tightly, the healing processes were observed and were histologically compared with each other after 2days, 4days, 1week, 2weeks, 4weeks. The results were obtained as follows : 1. In the two groups blood clots were observed as early as 2 and 4 days, and were resorbed at 1 week. 2. In the two groups moderate inflammation was observed as early as 2 and 4 days, decreased at 1 and 2 weeks, and disappeared at 4 weeks. 3. Junctional Epithelium migration was more significant in the control group, and was restrained by graft materials in the experimental group. 4. Features of connective tissue fiber attachment partially showed the parallel pattern in the two groups from 2 weeks, and entirely from 4weeks. 5. Anastomosis, between graft and connective tissue, appeared from 4 days in the experimental group and the border between them was not discriminated at 4weeks.